DuPont™ BAX® System Certified by AOAC for Real-Time L. monocytogenes Detection
The DuPont™ BAX® System Real-Time PCR Assay for L. monocytogenes has been certified as a Performance Tested Method? (PTM) by the AOAC Research Institute, a subsidiary of AOAC International in Rockville, Md. This next-generation test, which combines shorter, simpler sample preparation and faster real-time processing without sacrificing accuracy or reliability, has been validated on a variety of sample matrices, including frankfurters, cooked shrimp, spinach, queso fresco and environmental surfaces.
In addition to the 24LEB- and reference method media-based enrichment protocols published during the commercialization of the BAX® System assay in July 2014, this AOAC certification includes a new combined testing method from environmental samples developed by DuPont Nutrition & Health and FoodChek™ Systems Inc. of Calgary, Alberta, Canada. This new combined testing method includes a 20-hour, single-stage enrichment in FoodChek’s proprietary Actero™ Listeria Enrichment Media followed by rapid real-time processing with the DuPont™ BAX® System. The method allows for the detection of L. monocytogenes from environmental samples in about 22 hours, one of the fastest time-to-results available for the pathogen in the food safety industry today.