HR-1 Stress Test™—This test is designed to “red flag” products that have potential coliform and other Gram-negative contamination. It is done by stressing the product in a media containing selective bacterial inhibitors at a temperature (32°C) that favors the growth of coliform type (Gram-negative) bacteria. Using this test, products contaminated with coliform type bacteria can be identified in 18-22 hours by a change in color from purple to pink or white (Figure 1). A positive HR-1 test indicates a serious sanitation problem and virtually no chance for acceptable keeping quality.
HR-2 Shelf-Life Test™—This is a qualitative test used to predict shelf life based on the level of post-pasteurization contamination. As with the HR-1 test, the HR-2 involves stressing the product in a media containing selective microbial inhibitors at temperatures (70-72°F) that favor the growth of spoilage bacteria. A change in color from white to pink indicates the presence of psychrotrophic bacteria and the likelihood of shelf-life problems. However, interpretation of the test results also depends on the time it takes for the color change to occur (Figure 2). A color change in less than 24 hours indicates a potentially serious shelf-life problem while no color change in 48 hours represents the potential for excellent shelf-life (14 days or better at 45°F).
Negative results for both tests (HR-1 and HR-2) indicate effective sanitation practices on the pasteurized side and the potential for excellent keeping quality. However, if actual keeping quality (45°F for 14 days) is not consistent with the negative test results, spoilage is likely related to the presence of heat-resistant, spore-forming bacteria.
HR-3 Screening Test™—Unlike the HR-1 and HR-2 tests, which specifically indicate Gram-negative contamination, the HR-3 test is designed for screening raw and pasteurized milks for the presence of Gram-positive, spore-forming rods. As code life of fluid products has increased to beyond 14 days, these bacteria have become very important in determining quality. Not only do they survive pasteurization, but they may grow at sub-pasteurization temperatures of 130 to 145˚F and slowly at refrigerated temperatures. The HR-3 test detects presence of heat-resistant bacteria and enables plant operators to identify original sources of contamination such as individual farms or tankers. It is also useful in isolating unsanitary processing equipment. As with the other HR tests, the protocol uses a vivid color indication for positive samples. The presence of large Gram-positive rod shaped bacteria is confirmed by microscopic examination. The HR-3 test is simple to perform and gives timely results, usually within 14 hours (Figure 3).
By utilizing these test procedures, good control may be achieved for high quality products with superb shelf-life.
